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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Mar 6, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Single-Molecule Localization Microscopy in Eukaryotes.

Markus Sauer¹, Mike Heilemann²

¹Department of Biotechnology & Biophysics, Julius-Maximilian-University of Würzburg , 97074 Würzburg, Germany.

Chemical Reviews

|March 14, 2017

Summary

This summary is machine-generated.

Single-molecule localization microscopy (SMLM) offers molecular-level resolution for cellular imaging. This technique reveals protein organization and interactions, advancing our understanding of cell function.

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Area of Science:

Cell biology
Biophysics
Microscopy

Background:

Super-resolution fluorescence imaging techniques achieve resolutions beyond the diffraction limit of light.
Single-molecule localization microscopy (SMLM) enables visualization of cellular structures at the molecular level.

Purpose of the Study:

To review the contributions of SMLM to eukaryotic biology.
To highlight the extraction of quantitative biological data from SMLM images.

Main Methods:

Photoactivation or photoswitching of single fluorophores.
Precise determination of single-molecule positions.
Image reconstruction to achieve super-resolution.

Main Results:

SMLM provides molecule-resolved information and super-resolved images of cellular structures.
Quantitative analysis of protein distribution, organization, and interactions is possible.
Determination of protein numbers within cells and protein complex stoichiometry.

Conclusions:

SMLM provides unprecedented insights into cellular organization and function at the molecular level.
This technique is crucial for quantitative biology and understanding molecular mechanisms in cells.