Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.2K
CRISPR01:59

CRISPR

58.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.4K
Homologous Recombination02:31

Homologous Recombination

64.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct and indirect regulation of fetal globin transcript by RNA-binding protein IGF2BP1.

BloodĀ·2026
Same author

Genetic Medicine - Primed and Ready.

The New England journal of medicineĀ·2026
Same author

Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing.

Nature communicationsĀ·2026
Same author

Analyzing long-read CRISPR experiments with CRISPRLungo.

bioRxiv : the preprint server for biologyĀ·2025
Same author

Modular Platform for Therapeutic Drug Delivery Using Trifunctional Bio-Orthogonal Macromolecular Conjugates.

Bioconjugate chemistryĀ·2025
Same author

The XPO7-NPAT axis represents key vulnerabilities in TP53-mutated acute myeloid leukemia.

BloodĀ·2025

Related Experiment Video

Updated: Mar 6, 2026

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.7K

Functional interrogation of non-coding DNA through CRISPR genome editing.

Matthew C Canver1, Daniel E Bauer2, Stuart H Orkin3

  • 1Harvard Medical School, Boston, MA 02115, United States.

Methods (San Diego, Calif.)
|March 15, 2017
PubMed
Summary

CRISPR genome editing advances offer new ways to study non-coding DNA, which makes up most of the genome. This review covers CRISPR-based loss- and gain-of-function techniques for exploring these crucial genetic regions.

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.1K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.6K

Related Experiment Videos

Last Updated: Mar 6, 2026

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.7K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.1K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.6K

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Non-coding DNA comprises the vast majority of the genome, yet its study has lagged behind coding regions.
  • The evolution of genome editing technologies has created new opportunities for non-coding DNA research.

Purpose of the Study:

  • To review CRISPR-based loss-of-function and gain-of-function techniques for interrogating non-coding DNA.
  • To highlight the utility of CRISPR technology in advancing the study of the non-coding genome.

Main Methods:

  • Review of CRISPR-mediated loss-of-function strategies (sequence disruption, transcriptional repression).
  • Review of CRISPR-mediated gain-of-function approaches (sequence integration, transcriptional activation).

Main Results:

  • CRISPR technology provides a versatile toolbox for studying non-coding DNA.
  • Loss-of-function methods include direct sequence disruption or repression of transcription.
  • Gain-of-function methods involve sequence integration or activation of transcription.

Conclusions:

  • CRISPR-based techniques significantly enhance the ability to investigate non-coding DNA.
  • These methods are crucial for understanding the function and regulation of the majority of the genome.