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Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus
1Institut Jacques Monod, Paris, France.
Molecular & General Genetics : MGG
|November 1, 1987
Abstract:
We measured in Bacillus subtilis the recombination frequency between directly repeated 9 bp sequences flanking the transposon Tn5. Four different repeats were analyzed. They recombined with frequencies between 10(-7) and 10(-6) when they were carried on plasmids, and 150-1500 times less frequently when they were carried on the chromosome. We propose that this difference is due to the generation of single stranded DNA during plasmid replication.