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MiR-214 regulates oral cancer KB cell apoptosis through targeting RASSF5

T K Li1, K Yin2, Z Chen1

  • 1Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Genetics and Molecular Research : GMR
|March 15, 2017
PubMed

Insights

MicroRNA-214 (miR-214) is elevated in oral cancer, suppressing Ras association domain family member 5 (RASSF5) expression. This interaction promotes oral cancer cell apoptosis, suggesting miR-214 as a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Ras association domain family member 5 (RASSF5) induces apoptosis via FOXO3a phosphorylation and BIM activation.
  • MicroRNA-214 (miR-214) is overexpressed in oral cancer, with predicted binding to RASSF5 mRNA.
  • The regulatory role of miR-214 on RASSF5 in oral cancer pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the role of miR-214 in regulating RASSF5 expression within the context of oral cancer.
  • To elucidate the molecular mechanisms by which miR-214 influences oral cancer cell apoptosis.

Main Methods:

  • Analysis of miR-214 and RASSF5 expression in oral cancer tissues versus paracarcinoma tissues from 48 patients.
  • Dual luciferase reporter gene assay to confirm the interaction between miR-214 and RASSF5 3'-UTR.
  • In vitro studies using oral cancer KB cells treated with miR-214 inhibitors and RASSF5 expression vectors.
  • Assays for caspase 3 activity, cell apoptosis (flow cytometry), and protein expression (Western blot).

Main Results:

  • Significantly increased miR-214 and decreased RASSF5 expression were observed in oral cancer tissues.
  • Luciferase assays confirmed that miR-214 directly targets the 3'-UTR of RASSF5, inhibiting its expression.
  • Down-regulation of miR-214 or enhancement of RASSF5 expression led to increased FOXO3a phosphorylation, BIM expression, caspase 3 activity, and apoptosis in KB cells.

Conclusions:

  • miR-214 is upregulated and RASSF5 is downregulated in oral cancer.
  • miR-214 promotes oral cancer cell apoptosis by targeting RASSF5, subsequently affecting FOXO3a phosphorylation and BIM expression.
  • The miR-214/RASSF5 pathway represents a potential novel therapeutic target for oral cancer treatment.

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