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Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

A novel SERS-based magnetic aptasensor for prostate specific antigen assay with high sensitivity.

Kang Yang¹, Yongjun Hu¹, Ning Dong¹

¹MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.

Biosensors & Bioelectronics

|March 16, 2017

Summary

This study introduces a novel aptamer-based SERS sensor using magnetic nanoparticles for highly sensitive prostate specific antigen (PSA) detection. The sensor achieves a low limit of detection, showing promise for clinical applications in PSA diagnostics.

Keywords:

Aptamer Core-satellite assemblies Magnetic nanoparticles PSA SERS

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Last Updated: Mar 6, 2026

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Published on: June 13, 2010

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Area of Science:

Nanotechnology
Biomedical Engineering
Analytical Chemistry

Background:

Accurate prostate specific antigen (PSA) detection is crucial for diagnosing prostate cancer, particularly in challenging patient groups like the obese.
Existing detection methods may lack the required sensitivity or specificity for early diagnosis and monitoring.
Novel sensor technologies are needed to improve PSA detection accuracy and accessibility.

Purpose of the Study:

To develop and validate a novel aptamer-based Surface-Enhanced Raman Scattering (SERS) sensor for highly sensitive and accurate PSA detection.
To utilize magnetic nanoparticles (MNPs) core-Au nanoparticles (AuNPs) satellite assemblies for enhanced signal amplification and efficient separation.
To assess the sensor's performance, including its limit of detection (LOD) and applicability in human serum samples.

Main Methods:

Fabrication of a sensor using aptamer-modified MNPs and AuNPs forming core-satellite assemblies.
Exploitation of PSA-aptamer biorecognition to induce assembly dissolution and release of signal probes (AuNPs).
Magnetic separation of MNPs and subsequent SERS analysis of the supernatant to quantify PSA concentration.

Main Results:

The SERS signal intensity in the supernatant directly correlated with PSA concentration over a wide range.
Achieved a highly sensitive limit of detection (LOD) as low as 5.0 pg/mL for PSA.
Demonstrated excellent recovery rates, indicating the sensor's feasibility for detecting PSA in human serum samples.

Conclusions:

The novel aptamer-based SERS sensor offers accurate and highly sensitive PSA detection.
The use of MNP core-AuNP satellite assemblies provides an effective platform for signal amplification and sample preparation.
This sensor shows promising potential for future clinical applications in diagnosing and monitoring prostate cancer.