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Raphasemenate A-J: Ten phenylpropionates with antibacterial activity from Raphani Semen.

Meng-Qi Yan¹, Kang Yang¹, Rui-Tong Du¹

¹Key Laboratory of Basic and Application Research of Beiyao (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, People's Republic of China.

Bioorganic Chemistry

|November 9, 2025

Summary

Researchers identified new phenylpropionate compounds from Raphani Semen with significant antibacterial activity against Staphylococcus aureus. Compound 4 showed excellent efficacy and enhanced antibiotic action, with its mechanism involving key protein targets.

Keywords:

Antibacterial activity Phenylpropionates Raphani semen Raphasemenate a – J

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Published on: January 3, 2018

Area of Science:

Natural Product Chemistry
Pharmacology
Microbiology

Background:

Raphani Semen is a traditional medicine source.
Phenylpropionate compounds possess diverse biological activities.
Antibiotic resistance necessitates novel antibacterial agents.

Purpose of the Study:

To isolate and characterize new phenylpropionate compounds from Raphani Semen.
To evaluate the antibacterial activity of these compounds against Staphylococcus aureus.
To elucidate the antibacterial mechanism of action for promising compounds.

Main Methods:

Extraction and isolation of compounds from Raphani Semen.
Spectroscopic analyses (NMR, MS) for structural elucidation.
Antibacterial assays (MIC determination) and synergy testing.
Network pharmacology, molecular docking, and molecular dynamics simulations.

Main Results:

Ten new and ten known phenylpropionate compounds were identified.
Compound 4 exhibited potent inhibitory effects against Staphylococcus aureus.
Compound 4 demonstrated an additive effect with ampicillin sodium.
Network pharmacology predicted EGFR, GAPDH, and MMP9 as core targets for compound 4.

Conclusions:

Raphani Semen is a valuable source of phenylpropionate compounds with antibacterial potential.
Compound 4 represents a promising lead for developing new antibacterial strategies.
The identified targets provide insights into the mechanism of action, aiding future drug development.