Related Experiment Video
Updated: Mar 6, 2026
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
Published on: November 10, 2016
Analysis of Ethylene Receptors: Ethylene-Binding Assays
Brad M Binder1, G Eric Schaller2
1Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, M407 Walters Life Sciences, 1414 Cumberland Ave., Knoxville, TN, 37996, USA. bbinder@utk.edu.
Abstract:
Plant ethylene receptors bind ethylene with high affinity. Most of the characterization of ethylene binding to the receptors has been carried out using a radioligand-binding assay on functional receptors expressed in yeast. In this chapter, we describe methods for expressing ethylene receptors in yeast and conducting ethylene-binding assays on intact yeast and yeast membranes. The ethylene-binding assays can be modified to analyze ethylene binding to intact plants and other organisms as well as membranes isolated from any biological source.
