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Updated: Mar 6, 2026
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
An NIR-emitting lysosome-targeting probe with large Stokes shift via coupling cyanine and excited-state
Dipendra Dahal1, Lucas McDonald1, Xiaoman Bi1
1Department of Chemistry, University of Akron, Akron, Ohio 44325, USA. yp5@uakron.edu.
Abstract:
An NIR-emitting probe (λem ∼ 700 nm) with a large Stokes shift (Δλ ≈ 234 nm) is synthesized by using excited-state intramolecular proton transfer (ESIPT). The phenolic proton, which controls ESIPT, acts as a switch to give strong fluorescence at pH ≈ 5. The probe can selectively show lysosome organelles, therefore leading to a lysosome probe without exhibiting "an alkalinizing effect".
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