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A preparative method for the enzymatic 5'-monophosphorylation of nucleosides
1Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Analytical Biochemistry
|November 15, 1987
Abstract:
A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [gamma-32P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.