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Differential Gene Expression of Longan Under Simulated Acid Rain Stress
Shan Zheng1, Tengfei Pan1, Cuilan Ma1
1College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Abstract:
Differential gene expression profile was studied in Dimocarpus longan Lour. in response to treatments of simulated acid rain with pH 2.5, 3.5, and a control (pH 5.6) using differential display reverse transcription polymerase chain reaction (DDRT-PCR). Results showed that mRNA differential display conditions were optimized to find an expressed sequence tag (EST) related with acid rain stress. The potential encoding products had 80% similarity with a transcription initiation factor IIF of Gossypium raimondii and 81% similarity with a protein product of Theobroma cacao. This fragment is the transcription factor activated by second messenger substances in longan leaves after signal perception of acid rain.
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