Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scalable genotyping in fixed transcriptomes resolves clonal heterogeneity via single-cell sequencing.

bioRxiv : the preprint server for biology·2026
Same author

Impact of Continuous Glucose Monitoring on Clinical Outcomes and Productivity Losses Among People Living With Type 2 Diabetes Not Using Insulin From an Employer's Perspective.

Journal of diabetes science and technology·2026
Same author

Impact of Continuous Glucose Monitor on Health Care Resource Utilization in Adults with Type 2 Diabetes Managed by Noninsulin Therapies.

Diabetes technology & therapeutics·2026
Same author

Insights into the structure and modulation of human TWIK-2.

Nature communications·2026
Same author

Exploring patient preferences regarding the use of combination therapy with endothelin receptor antagonist (ERA) + phosphodiesterase-5 inhibitors (PDE5i).

JHLT open·2025
Same author

Exploring Providers' Behaviors, Attitudes, and Preferences on the Treatment of Pulmonary Arterial Hypertension With Endothelin Receptor Antagonist (ERA) + Phosphodiesterase-5 Inhibitors (PDE5i).

Pulmonary circulation·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Mar 6, 2026

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

15.5K

Functionalized Vesicles by Microfluidic Device.

Derek Vallejo1, Shih-Hui Lee1, Abraham Lee2

  • 1Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92697 2715, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 17, 2017
PubMed
Summary
This summary is machine-generated.

Giant unilamellar vesicles (GUVs) are produced using microfluidics for biosensor development. These lipid vesicles offer a stable environment for membrane proteins, enabling sensitive analyte detection.

Keywords:
Artificial cellBiosensorDewettingGiant unilamellar vesicleMicrofluidicsSolvent extraction

More Related Videos

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.5K
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.9K

Related Experiment Videos

Last Updated: Mar 6, 2026

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

15.5K
Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.5K
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.9K

Area of Science:

  • Biotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Lipid vesicles are increasingly utilized as platforms for biosensor development.
  • Their selective permeable membrane provides an ideal environment for membrane protein biosensing elements, preserving native structure and function.
  • Membrane fluidity facilitates conformational changes and analyte interactions.

Purpose of the Study:

  • To present two microfluidic methods for producing giant unilamellar vesicles (GUVs) for biosensor applications.
  • To demonstrate the utility of water-in-oil-in-water (W/O/W) double emulsion templates for GUV fabrication.
  • To introduce strategies for integrating sensing elements and membrane proteins onto GUVs.

Main Methods:

  • Production of GUVs using microfluidic devices and W/O/W double emulsion templates.
  • Nonvolatile oil phase removal via ethanol extraction or interfacial tension alteration.
  • Development of methods for incorporating sensing elements and membrane protein pores.

Main Results:

  • Successful generation of GUVs using two distinct microfluidic-based methods.
  • Demonstration of oil phase removal techniques, yielding functional lipid bilayers.
  • Establishment of pathways for integrating biosensing components onto the vesicles.

Conclusions:

  • Microfluidic production of GUVs offers a robust platform for advanced biosensor design.
  • The presented methods facilitate the creation of vesicles suitable for housing membrane proteins.
  • These GUVs serve as a foundation for developing novel biosensing devices with enhanced sensitivity and specificity.