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Updated: Mar 6, 2026
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
A new LC-MS/MS bioanalytical method for atenolol in human plasma and milk
Ei Mon Phyo Lwin1, Cobus Gerber1, Yunmei Song1
1School of Pharmacy & Medical Sciences, Reid Building, City East Campus, University of South Australia, Adelaide, SA 5000, Australia.
Aim:
A new sensitive LC-MS/MS method for the quantification of atenolol in human plasma and milk has been developed for clinical lactation studies.
Methods & Results:
Atenolol and the internal standard, phenazone, were extracted from biological matrices by protein precipitation. A Phenomenex® C-18 column and gradient chromatographic conditions were used for separation of the analyte, followed by detection with MS. Stability of samples was confirmed for atenolol in human plasma and milk for up to 3 months. Linearity range of 1-800 ng/ml (r2 = 0.9995), the precision within 15% CV and the recovery of the analyte (80-100% range) were achieved.
Conclusion:
A new validated analytical method for atenolol in plasma and milk was developed.
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