Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.8K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.0K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.5K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

2.7K
2.7K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.8K
Overview
80.8K
Transgenic Organisms00:53

Transgenic Organisms

34.1K
Overview
34.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A minimal transcription factor network is sufficient to drive paclitaxel biosynthesis.

Science advances·2026
Same author

A Key Role for S-Nitrosylation in Immune Regulation and Development in the Liverwort Marchantia polymorpha.

Journal of experimental botany·2026
Same author

Dynamic basal ganglia output signals license and suppress forelimb movements.

Nature·2025
Same author

Trans-eQTL hotspots shape complex traits by modulating cellular states.

Cell genomics·2025
Same author

Recent advances in designing synthetic plant regulatory modules.

Frontiers in plant science·2025
Same author

Interorgan, intraorgan and interplant communication mediated by nitric oxide and related species.

The New phytologist·2024
Same journal

Enzymes for CO<sub>2</sub> fixation: Discovery, engineering, and applications.

Biotechnology advances·2026
Same journal

Technological advances in extrachromosomal circular DNA detection.

Biotechnology advances·2026
Same journal

Codon compression and novel codon creation for multiplex non-canonical amino acid incorporation.

Biotechnology advances·2026
Same journal

Toward next-generation biosurfactants: Engineering rhamnolipid production from safe chassis design to scalable bioprocessing.

Biotechnology advances·2026
Same journal

Advances and challenges in alternative proteins: From biotechnology to sustainable food production.

Biotechnology advances·2026
Same journal

Recent advances in the microbial production of L-arginine and its derivatives using engineering Corynebacterium glutamicum and Escherichia coli.

Biotechnology advances·2026
See all related articles

Related Experiment Video

Updated: Mar 6, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.1K

Towards combinatorial transcriptional engineering.

Rajesh Mehrotra1, Kaushik Renganaath1, Harsh Kanodia1

  • 1Department of Biological Sciences, Birla Institute of Technology and Science (BITS) Pilani, Pilani 333031, Rajasthan, India.

Biotechnology Advances
|March 17, 2017
PubMed
Summary
This summary is machine-generated.

Synthetic transcriptional engineering enables designing genetic circuits using modular parts. This approach allows for predictable control and diverse applications in research and biotechnology.

Keywords:
CRISPRGene expressionPromotersSynthetic biologyTaleTranscription factorsTranscriptional engineering

More Related Videos

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

9.6K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.9K

Related Experiment Videos

Last Updated: Mar 6, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.1K
An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

9.6K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.9K

Area of Science:

  • Synthetic biology
  • Genetic engineering
  • Molecular biology

Background:

  • Transcriptional units offer modularity for designing biological systems.
  • Customizable regulatory circuits have broad research and applied potential.
  • Synthetic transcriptional engineering aims to build complex genetic cascades.

Purpose of the Study:

  • To discuss the core principles of synthetic transcriptional engineering.
  • To review technologies for promoter and transcriptional factor engineering.
  • To explore programming inducibility into synthetic genetic modules.

Main Methods:

  • Review of current technologies in transcriptional engineering.
  • Analysis of promoter and transcriptional factor engineering strategies.
  • Discussion of methods for controlling gene expression inducibility.

Main Results:

  • Modular design allows for predictable input-output characteristics.
  • Synthetic transcriptional engineering offers diverse applications.
  • Rational combinatorial engineering is key for future development.

Conclusions:

  • Synthetic transcriptional engineering is a powerful tool for creating custom genetic circuits.
  • Understanding and engineering promoters, transcription factors, and inducibility are crucial.
  • A combinatorial engineering approach promises significant advancements in the field.