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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Towards combinatorial transcriptional engineering.

Rajesh Mehrotra1, Kaushik Renganaath1, Harsh Kanodia1

  • 1Department of Biological Sciences, Birla Institute of Technology and Science (BITS) Pilani, Pilani 333031, Rajasthan, India.

Biotechnology Advances
|March 17, 2017
PubMed
Summary
This summary is machine-generated.

Synthetic transcriptional engineering enables designing genetic circuits using modular parts. This approach allows for predictable control and diverse applications in research and biotechnology.

Keywords:
CRISPRGene expressionPromotersSynthetic biologyTaleTranscription factorsTranscriptional engineering

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Area of Science:

  • Synthetic biology
  • Genetic engineering
  • Molecular biology

Background:

  • Transcriptional units offer modularity for designing biological systems.
  • Customizable regulatory circuits have broad research and applied potential.
  • Synthetic transcriptional engineering aims to build complex genetic cascades.

Purpose of the Study:

  • To discuss the core principles of synthetic transcriptional engineering.
  • To review technologies for promoter and transcriptional factor engineering.
  • To explore programming inducibility into synthetic genetic modules.

Main Methods:

  • Review of current technologies in transcriptional engineering.
  • Analysis of promoter and transcriptional factor engineering strategies.
  • Discussion of methods for controlling gene expression inducibility.

Main Results:

  • Modular design allows for predictable input-output characteristics.
  • Synthetic transcriptional engineering offers diverse applications.
  • Rational combinatorial engineering is key for future development.

Conclusions:

  • Synthetic transcriptional engineering is a powerful tool for creating custom genetic circuits.
  • Understanding and engineering promoters, transcription factors, and inducibility are crucial.
  • A combinatorial engineering approach promises significant advancements in the field.