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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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Coded Apertures in Mass Spectrometry.

Jason J Amsden¹, Michael E Gehm¹, Zachary E Russell²

¹Department of Electrical and Computer Engineering, Duke University, Durham, North Carolina 27708;

Annual Review of Analytical Chemistry (Palo Alto, Calif.)

|March 17, 2017

Summary

This summary is machine-generated.

Coded apertures in mass spectrometry enhance instrument throughput by over 10x without sacrificing resolution. This innovation improves sensitivity for detecting low analyte concentrations, advancing analytical capabilities.

Keywords:

Mattauch-Herzog coded apertures computational sensing cycloidal mass analyzer miniaturization sector mass spectrometry

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Area of Science:

Analytical Chemistry
Spectrometry
Instrument Development

Background:

Conventional mass spectrometry instruments face a trade-off between throughput and resolution.
This limitation hinders the detection of analytes at low concentrations.
Existing technologies struggle to simultaneously increase signal acquisition speed and maintain spectral accuracy.

Purpose of the Study:

To review the development and application of coded apertures in mass spectrometry.
To explore how coded apertures overcome the throughput-resolution limitations of conventional instruments.
To discuss the potential impact of coded apertures on analytical sensitivity and selectivity.

Main Methods:

Implementation of coded apertures within mass spectrometry instrumentation.
Demonstration of both one- and two-dimensional (2D) coding strategies.
Evaluation of instrument modifications required for integrating coded apertures.

Main Results:

Coded apertures demonstrated over a tenfold increase in throughput in a 90-degree magnetic sector.
No loss in spectral resolution was observed with the use of coded apertures.
Enhanced throughput improves signal-to-noise ratio, boosting sensitivity for low-concentration analytes.

Conclusions:

Coded apertures offer a significant advancement in mass spectrometry, breaking the traditional throughput-resolution barrier.
The technology promises improved sensitivity and selectivity, crucial for complex analytical challenges.
Further research and instrument development are needed to fully realize the potential of coded apertures in mass spectrometry.