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Updated: Mar 6, 2026

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

Imaging and Optically Manipulating Neuronal Ensembles.

Luis Carrillo-Reid^1,2, Weijian Yang^1,2, Jae-Eun Kang Miller^1,2

¹NeuroTechnology Center, Columbia University, New York, NY 10027.

Annual Review of Biophysics

|March 17, 2017

Summary

This summary is machine-generated.

Researchers review advanced optical and computational methods for studying neuronal ensembles, the building blocks of neural circuits. These techniques can decode the neural code and offer new therapeutic strategies for brain disorders.

Keywords:

calcium optogenetics two-photon

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Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

Area of Science:

Neuroscience
Computational Neuroscience
Optical Imaging

Background:

Neural activity relies on spatiotemporal patterns within neuronal populations.
Neuronal ensembles are key functional units within neural circuits.
Understanding these ensembles is crucial for deciphering the neural code.

Purpose of the Study:

To review advanced optical and computational methods for studying neuronal ensembles.
To discuss the application of these methods in the mammalian cortex.
To explore the potential of these techniques for understanding brain function and disease.

Main Methods:

Two-photon calcium imaging for detecting and characterizing neuronal ensembles in 3D.
Two-photon optogenetics for manipulating neuronal ensembles in vivo and in vitro.
Computational approaches for analyzing complex neural activity patterns.

Main Results:

Demonstrated the existence of neuronal ensembles in spontaneous and evoked cortical activity.
Showcased the ability to artificially imprint and selectively recall neuronal ensembles in behaving animals.
Highlighted the 3D detection and manipulation capabilities of the reviewed methods.

Conclusions:

These advanced methods are powerful tools for deciphering the neural code.
The techniques offer potential for understanding neurological and mental diseases.
Future applications include designing novel therapeutic strategies for brain disorders.