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Proteome Analysis Using Isobaric Tags for Relative and Absolute Analysis Quantitation (iTRAQ) Reveals Alterations in
Tong Xing1, Chong Wang1, Xue Zhao1
1Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agricultural University , Nanjing 210095, China.
Journal of Agricultural and Food Chemistry
|March 18, 2017
Summary
High-temperature transport induces stress in broilers, altering muscle protein profiles and leading to pale, soft, and exudative (PSE)-like meat quality. Proteomic analysis identified key protein changes contributing to this stress response.
Area of Science:
- Animal Science
- Proteomics
- Food Science
Background:
- Transportation stress negatively impacts broiler meat quality, leading to pale, soft, and exudative (PSE) meat.
- Understanding the molecular mechanisms behind PSE meat development is crucial for improving poultry production.
Purpose of the Study:
- To investigate proteomic changes in broiler Pectoralis major muscles exhibiting PSE-like characteristics after transport under high-temperature conditions.
- To identify specific proteins and pathways involved in the stress response and their correlation with meat quality defects.
Main Methods:
- Isobaric tags for relative and absolute quantitation (iTRAQ) was employed for proteomic analysis.
- Broilers were subjected to transportation stress (0.5 h) under high-temperature conditions.
- Plasma stress indicators, muscle microstructure, and proteome were analyzed in normal and PSE-like muscle samples.
Main Results:
- Transported broilers developing PSE-like meat showed elevated plasma stress indicators and altered muscle microstructure (looser network, larger intercellular spaces).
- iTRAQ analysis identified 29 differentially expressed proteins in PSE-like muscles, associated with protein turnover, signal transduction, stress response, calcium handling, and metabolism.
- Significant alterations were observed in proteins related to glycolysis, calcium signaling, and molecular chaperones.
Conclusions:
- High-temperature transport stress significantly impacts broiler muscle proteome, contributing to PSE meat quality.
- Specific protein pathways, including glycolysis and calcium signaling, are implicated in the development of stress-induced meat quality defects.
- Proteomic insights enhance the understanding of the molecular basis of meat quality changes in response to environmental stressors.
