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Updated: Mar 6, 2026
Primary Cell Cultures from Drosophila Gastrula Embryos
Published on: February 28, 2011
In vitro development of theDrosophila chorion in a chemically defined organ culture medium
William H Petri1, Mihalis N Mindrinos1, Mary F Lombard1
1Biology Department, Boston College, 02167, Chestnut Hill, Massachusetts, USA.
Abstract:
TheDrosophila chorion is produced normally in isolated follicles in Robb's chemically defined culture medium. The complex architecture of the shell developed in vitro from follicles as young as early stage 10 is completely normal morphologically. In addition, the time required for in vitro development closely approximates that observed for in vivo development. Comparisons of insect culture media developed by Robb, Grace, Schneider, and Echalier show large variations in their ability to supportDrosophila chorion development.
