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Ecdysteroids induce morphological changes in continuous cell lines of Lepidoptera.
Naomi Kislev1,2, Israel Segal3, Marvin Edelman1
1Department of Plant Geneties, The Weizmann Institute of Science, 76100, Rehovot, Israel.
Wilhelm Roux'S Archives of Developmental Biology
|March 18, 2017
Summary
Ecdysteroids induce morphological changes in some Lepidoptera cell lines, with 20-hydroxyecdysone being more potent. Different cell lines exhibit varying responsiveness to these insect hormones.
Area of Science:
- Insect cell biology
- Endocrinology
- Molecular biology
Background:
- Continuous insect cell lines are valuable models for studying cellular responses.
- Ecdysteroids are crucial insect hormones regulating development and metamorphosis.
- Understanding ecdysteroid action in cell lines aids in insect physiology research.
Purpose of the Study:
- To investigate the effects of ecdysone and 20-hydroxyecdysone on continuous Lepidoptera cell lines.
- To compare the responsiveness of different insect cell lines to ecdysteroids.
- To characterize the dose-dependent effects of ecdysteroids on cell morphology.
Main Methods:
- Treatment of Trichoplusia ni (TN-368) and Spodoptera littoralis (HPB-SL-26) cell lines with ecdysone and 20-hydroxyecdysone.
- Observation and documentation of morphological changes in treated cells.
- Comparison of responses across different cell lines, including S. littoralis (UIV-SL-573) and S. frugiperda (IPLB-SF-21).
Main Results:
- Ecdysone and 20-hydroxyecdysone induced cell aggregation, elongation, and process extension in TN-368 and HPB-SL-26 cells.
- The observed morphological changes were dose-dependent.
- 20-hydroxyecdysone demonstrated significantly higher activity than ecdysone.
- UIV-SL-573 and IPLB-SF-21 cell lines showed markedly lower responsiveness to ecdysteroids.
Conclusions:
- Continuous Lepidoptera cell lines exhibit differential sensitivity to ecdysteroids.
- TN-368 and HPB-SL-26 cells are suitable models for studying ecdysteroid-induced morphological changes.
- Ecdysteroid potency varies, with 20-hydroxyecdysone being a stronger inducer of cellular responses.

