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Biosynthesis of Polysaccharides

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Biological activity of the vegetalizing factor: Decrease after coupling to polysaccharide matrix and enzymatic

Jochen Born¹, Horst Grunz¹, Heinz Tiedemann¹

¹Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.

Wilhelm Roux'S Archives of Developmental Biology

|March 18, 2017

Summary

The vegetalizing factor requires internalization for biological activity, unlike the neuralizing factor which acts on the cell surface. Binding to certain matrices inactivates the vegetalizing factor, suggesting its intracellular mechanism.

Keywords:

Biological activity Immobilized inducing factors

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Area of Science:

Developmental Biology
Molecular Biology
Cell Biology

Background:

Understanding the mechanisms of embryonic development is crucial.
Factors influencing cell differentiation, such as vegetalizing and neuralizing factors, play key roles.
The precise mode of action for these factors at the cellular level requires elucidation.

Purpose of the Study:

To investigate the mechanism of action of vegetalizing and neuralizing factors.
To determine if these factors interact with the cell surface or require internalization.
To assess the impact of matrix binding on factor activity.

Main Methods:

Covalent coupling of factors to different matrices (CNBr-Sepharose, CH-Sepharose, Sephadex).
Assessing biological activity after binding and recovery.
Enzymatic degradation of matrices (dextranase) to recover active factor.

Main Results:

Covalent coupling to CNBr-Sepharose reduced vegetalizing factor activity.
Covalent coupling to CH-Sepharose inactivated vegetalizing factor but not neuralizing factor.
Binding to Sephadex reduced vegetalizing factor activity, with full recovery after dextranase treatment.

Conclusions:

Neuralizing factor likely acts on the ectoderm cell surface.
Vegetalizing factor probably requires internalization to exert its biological activity.
Matrix binding experiments provide insights into the distinct mechanisms of these developmental factors.