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Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
Published on: May 29, 2018
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Transgenic markers for mammalian chimeras
James Alexander Thomson1, Davor Solter1
1The Wistar Institute, 36th at Spruce Street, 19104, Philadelphia, PA, USA.
Abstract:
We describe the construction of aggregation chimeras between normal and transgenic embryos containing multiple copies of mouse β-globin genes. The transgenic component of the chimeras is then detected in tissue sections by a DNA-DNA in situ hybridization technique, using a biotinylated DNA β-globin probe and an avidin-linked alkaline phosphatase detection system. The general advantages of transgenic markers for chimeras are discussed.
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