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Tissue plasminogen activator mRNA in murine tissues
1Department of Microbiology, State University of New York, Stony Brook 11794.
FEBS Letters
|February 29, 1988
Summary
Researchers identified tissue-type plasminogen activator (tPA) mRNA in many mouse tissues. Urokinase-type plasminogen activator (uPA) mRNA was found in fewer tissues, with tPA mRNA containing complete functional domains.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- Mammals possess two key enzymes: urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA).
- These enzymes are crucial for converting plasminogen into plasmin, a vital process in the body.
Purpose of the Study:
- To investigate the distribution and characteristics of uPA and tPA messenger RNAs (mRNAs) in various murine tissues.
- To confirm the integrity of the detected tPA mRNA sequences.
Main Methods:
- Utilized complementary DNA (cDNA) probes to detect and quantify uPA and tPA mRNAs.
- Employed an S1 nuclease assay to verify the completeness of the tPA mRNA sequences.
Main Results:
- Tissue-type plasminogen activator mRNA was found to be widely distributed across a broad range of murine tissues.
- In contrast, urokinase-type plasminogen activator mRNA accumulation was limited to a select few tissues.
- The S1 nuclease assay confirmed that the detected tPA mRNA included complete coding sequences for non-protease, finger, growth-factor, and kringle domains.
Conclusions:
- Demonstrates a differential expression pattern of tPA and uPA mRNAs in murine tissues.
- Highlights the presence of fully functional tPA mRNA in various tissues, suggesting diverse roles.