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Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering
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Recombinant Allergen Production in E. coli.

Changqi Liu1, LeAnna N Willison2, Shridhar K Sathe3

  • 1School of Exercise and Nutritional Sciences, San Diego State University, 308 ENS Bldg., 5500 Campanile Drive, San Diego, CA, 92182-7251, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2017
PubMed
Summary
This summary is machine-generated.

This study details protocols for producing recombinant protein allergens. These allergens are crucial for allergy research, diagnosis, and understanding immune responses.

Keywords:
AllergyFood allergenRecombinant allergen

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Recombinant protein allergens are vital tools in allergy research.
  • Applications include allergy studies, diagnosis, and epitope mapping.

Purpose of the Study:

  • To describe detailed protocols for the production of recombinant allergens.
  • To provide a reproducible method for generating these essential research components.

Main Methods:

  • Messenger RNA (mRNA) isolation from target tissues.
  • Complementary DNA (cDNA) library construction.
  • Polymerase Chain Reaction (PCR) amplification and sequencing of allergen genes.
  • Cloning into expression vectors and expression in Escherichia coli.
  • Purification of recombinant proteins from cell lysates.

Main Results:

  • Established protocols for recombinant allergen production.
  • Demonstrated the feasibility of expressing and purifying functional recombinant allergens.

Conclusions:

  • The described methods enable reliable production of recombinant allergens.
  • These recombinant allergens are suitable for various applications in allergy research and diagnostics.