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Related Experiment Video

Updated: Mar 6, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

The case for applying tissue engineering methodologies to instruct human organoid morphogenesis.

Carlos R Marti-Figueroa¹, Randolph S Ashton¹

¹Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, United States.

Acta Biomaterialia

|March 20, 2017

Summary

This summary is machine-generated.

Tissue engineering can guide human pluripotent stem cell (hPSC)-derived organoid development. This approach aims to create standardized, biomimetic 3D organoids for research and potential transplantation.

Keywords:

3-D printing Bioprinting Click chemistry Hydrogels Morphogen gradients Organoid morphogenesis Photochemistry Sacrificial molding Tissue cytoarchitecture Tissue morphology

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Area of Science:

Biomedical Engineering
Developmental Biology
Stem Cell Biology

Background:

Human pluripotent stem cell (hPSC)-derived 3D organoids offer biomimetic in vitro models for studying development and disease.
Current organoid protocols rely on spontaneous morphogenesis, leading to macroscale inconsistencies in morphology, cytoarchitecture, and cellular composition.
These limitations hinder the standardization and application of organoids for research and potential therapeutic uses.

Purpose of the Study:

To review tissue engineering methodologies for instructing multiscale, 3D organoid morphogenesis.
To explore how integrating tissue engineering with organoid derivation can overcome current limitations.
To harness organoid morphogenesis for engineering functional human tissues with biomimetic anatomy and physiology.

Main Methods:

Review of existing tissue engineering strategies applicable to organoid development.
Discussion of methods to control microscale morphogenesis for macroscale biomimicry.
Exploration of technological mergers between tissue engineering and organoid derivation protocols.

Main Results:

Tissue engineering approaches offer potential for precise control over organoid morphogenesis at multiple scales.
Standardized in vitro morphogenesis can yield organoids with improved biomimicry in morphology, cytoarchitecture, and cellular composition.
This integration is crucial for advancing organoid technology towards functional tissue engineering.

Conclusions:

Integrating tissue engineering with hPSC-derived organoid protocols is essential for achieving controlled, biomimetic morphogenesis.
This synergy will enable the development of 'next-generation' organoids for advanced research and potential organ transplantation.
Harnessing these methodologies will unlock the full potential of organoids in creating anatomically correct and physiologically relevant human tissue models.