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Updated: Mar 6, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
Published on: February 18, 2022
Expression of a MORN repeat protein from Euplotes octocarinatus requires a +1 programmed ribosomal frameshifting
Lili Wei1, Xuemei Zhao1, Ruanlin Wang1
1a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Abstract:
Analysis of transcriptome revealed that a membrane occupation and recognition nexus (MORN) repeat protein-encoding gene of Euplotes octocarinatus (Eo-morn-9-31) was a candidate for programmed +1 ribosomal frameshifting (+1 PRF). In this study, a dual-luciferase assay was performed to detect its expression. The result showed that the MORN repeat protein (Eo-MORN-9-31) could be produced by the +1 PRF event during the process of translation in yeast and the frameshifting efficiency was about 4-5%. We further confirmed its reality by western blot and mass spectrometry. This study provided experimental evidence indicating that the expression of the Eo-MORN-9-31 of E. octocarinatus required the +1 PRF.
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