Three-dimensional ultrashort echo time cones T_1ρ (3D UTE-cones-T_1ρ ) imaging

We report a novel three-dimensional (3D) ultrashort echo time (UTE) sequence employing Cones trajectory and T_1ρ preparation (UTE-Cones-T_1ρ ) for quantitative T_1ρ assessment of short T₂ tissues in the musculoskeletal system. A basic 3D UTE-Cones sequence was combined with a spin-locking preparation pulse for T_1ρ contrast. A relatively short TR was used to decrease the scan time, which required T₁ measurement and compensation using 3D UTE-Cones data acquisitions with variable TRs. Another strategy to reduce the total scan time was to acquire multiple Cones spokes (N_sp ) after each T_1ρ preparation and fat saturation. Four spin-locking times (TSL = 0-20 ms) were acquired over 12 min, plus another 7 min for T₁ measurement. The 3D UTE-Cones-T_1ρ sequence was compared with a two-dimensional (2D) spiral-T_1ρ sequence for the imaging of a spherical CuSO₄ phantom and ex vivo meniscus and tendon specimens, as well as the knee and ankle joints of healthy volunteers, using a clinical 3-T scanner. The CuSO₄ phantom showed a T_1ρ value of 76.5 ± 1.6 ms with the 2D spiral-T_1ρ sequence, as well as 85.7 ± 3.6 and 89.2 ± 1.4 ms for the 3D UTE-Cones-T_1ρ sequences with N_sp of 1 and 5, respectively. The 3D UTE-Cones-T_1ρ sequence provided shorter T_1ρ values for the bovine meniscus sample relative to the 2D spiral-T_1ρ sequence (10-12 ms versus 16 ms, respectively). The cadaveric human Achilles tendon sample could only be imaged with the 3D UTE-Cones-T_1ρ sequence (T_1ρ  = 4.0 ± 0.9 ms), with the 2D spiral-T_1ρ sequence demonstrating near-zero signal intensity. Human studies yielded T_1ρ values of 36.1 ± 2.9, 18.3 ± 3.9 and 3.1 ± 0.4 ms for articular cartilage, meniscus and the Achilles tendon, respectively. The 3D UTE-Cones-T_1ρ sequence allows volumetric T_1ρ measurement of short T₂ tissues in vivo.