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Construction of cosmid genomic libraries for the normal and myopathic Syrian hamsters
J D McCully1, M A Jandreski, J Liew
1Department of Clinical Biochemistry, Banting Institute, University of Toronto, Ont., Canada.
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|November 1, 1987
Abstract:
Cosmid genomic libraries from both normal and myopathic Syrian hamsters have been constructed. MboI was used to generate 35- to 50-kilobase DNA fragments which were isolated from a 5-25% NaCl gradient. The 35- to 50-kilobase DNA fragments were ligated to the cosmid vector pCV108 and packaged into Escherichia coli DK1. Approximately 3 X 10(5) - 4 X 10(5) clones were obtained per microgram of ligated DNA. Thirteen clones have been isolated from 2 X 10(5) colonies using a cardiac myosin heavy chain clone as a probe. Restriction maps of two of these clones are presented here.