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Related Concept Videos

Gastrulation01:56

Gastrulation

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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
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Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development
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Par3 in chick lens placode development.

Maraysa de Oliveira Melo1, Ricardo Moraes Borges1, Chao Yun Irene Yan1

  • 1Department of Cell and Developmental Biology, Institute for Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, São Paulo, SP, 05508-900, Brazil.

Genesis (New York, N.Y. : 2000)
|March 21, 2017
PubMed
Summary
This summary is machine-generated.

The polarity protein PAR3 and its phosphorylation state are crucial for establishing the actin network in the developing chick lens placode. This finding sheds light on the molecular mechanisms governing early eye development.

Keywords:
Avianearly developmentorganism processplacodesprocesssignalingtissue

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Ophthalmology

Background:

  • The lens epithelium transforms into a pseudostratified lens placode during eye development.
  • Apical actin network formation causes cell narrowing in the lens placode.
  • Region-specific signaling mechanisms controlling actin network formation are largely unknown.

Purpose of the Study:

  • Investigate the role of the polarity protein PAR3 in chick lens placode development.
  • Examine the impact of PAR3's Threonine 833 (T833) phosphorylation site on aPKC recruitment and actin network establishment.

Main Methods:

  • Overexpression of wild-type PAR3 in chick lens placode cells.
  • Analysis of aPKC recruitment to basolateral membranes.
  • Assessment of ectopic actin spot induction and its dependence on T833 residue charge.

Main Results:

  • Overexpression of wild-type PAR3 recruited aPKC and actin clusters to basolateral membranes.
  • aPKC recruitment was dependent on the charge of the residue replacing T833.
  • Ectopic actin spot induction was independent of the T833 residue's charge.

Conclusions:

  • PAR3 plays a key role in recruiting aPKC to the basolateral membrane.
  • The phosphorylation state of T833 in PAR3 influences aPKC recruitment but not actin network formation.
  • These findings contribute to understanding the molecular basis of lens development and actin cytoskeleton regulation.