Related Experiment Video
Updated: Mar 6, 2026
Live-imaging of the Drosophila Pupal Eye
Published on: January 12, 2015
5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in
Yu Shan Huang1, Hui Yu Ku2,3, Yun Chi Tsai1
1Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
Abstract:
5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body. Furthermore, analysis of the en-expression pattern in the EADP provided strong evidence that the EADP is derived from one of the en-expressing head segments.
