Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Shaping neutrophil morphology and function: the importance of a segmented nucleus.

Nature reviews. Immunology·2026
Same author

In vivo cytokine adsorption reveals distinct neutrophil mobilization pathways during experimental human endotoxemia.

Journal of innate immunity·2026
Same author

A job exposure matrix for occupational exposure to airborne micro and nanoplastics (PlastiXJEM®️) and associations with respiratory outcomes.

Environmental research·2026
Same author

Micro- and nanoplastic exposure, immune cell activation, and lung function in young adults.

Microplastics and nanoplastics·2026
Same author

Letter to the editor - Early post lung transplant neutrophil dynamics within 72 h.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2026
Same author

Increased Bacterial Load per Neutrophil Reduces Intracellular Killing Capacity.

Journal of innate immunity·2026

Related Experiment Video

Updated: Mar 5, 2026

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.9K

Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis.

Erinke van Grinsven1, Chloé Prunier2, Nienke Vrisekoop1

  • 1Department of Respiratory Medicine, Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|March 22, 2017
PubMed
Summary

Two-photon intravital microscopy (2P-IVM) enables high-resolution in vivo imaging of dynamic cellular processes. This guide details four 2P-IVM methods to help researchers choose and set up the optimal approach for their studies.

Keywords:
ExplantImaging windowSkinSurgical exposureTwo-photon intravital microscopy

More Related Videos

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
09:00

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

19.7K
Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment
08:24

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

Published on: June 12, 2016

8.7K

Related Experiment Videos

Last Updated: Mar 5, 2026

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.9K
Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
09:00

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

19.7K
Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment
08:24

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

Published on: June 12, 2016

8.7K

Area of Science:

  • * Biomedical imaging
  • * Cell biology
  • * In vivo studies

Background:

  • * Dynamic cellular processes (migration, proliferation, signaling, interactions) occur in complex environments.
  • * Studying these processes in living animals is crucial for understanding biological functions.
  • * Two-photon intravital microscopy (2P-IVM) offers subcellular resolution for in vivo visualization.

Purpose of the Study:

  • * To provide a comprehensive guide for setting up and utilizing 2P-IVM.
  • * To compare and contrast four distinct 2P-IVM methodologies.
  • * To aid researchers in selecting the most appropriate 2P-IVM technique.

Main Methods:

  • * Detailed protocols for tissue explant imaging.
  • * Methodologies for skin imaging in vivo.
  • * Techniques for surgical exposure imaging.
  • * Procedures for multi-day window imaging.

Main Results:

  • * In-depth comparison of four 2P-IVM methods: tissue explant, skin, surgical exposure, and multi-day window imaging.
  • * Discussion of practical considerations and setup for each method.
  • * Mention of alternative solutions for advanced 2P-IVM applications.

Conclusions:

  • * The protocol serves as a decision-making guide for selecting the appropriate 2P-IVM method.
  • * Enables researchers to effectively set up and implement chosen 2P-IVM techniques.
  • * Facilitates advanced in vivo imaging of dynamic cellular events.