Related Experiment Video
Updated: Mar 5, 2026
Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
Published on: July 14, 2023
Bone regeneration enhancement by ultra-violet (UV) treatment for uHA/PLLA absorbable mesh
Hiroumi Ikawa1, Akinori Moroi1, Kunio Yoshizawa1
1Department of Oral & Maxillofacial Surgery (Chief Professor: Koichiro Ueki), Division of Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, Japan.
Purpose:
The purpose of this study was to evaluate the effect of bone regeneration enhancement by ultra-violet (UV) treatment of an unsintered hydroxyapatite (u-HA)/poly-l-lactic acid (PLLA) mesh in rabbitnasal bone.
Materials And Methods:
Thirty adult male Japanese white rabbits (12-16 weeks, 2.5-3.0 kg) were used in this study. After incising along the nasal bone, 6 × 6 × depth 1 mm two bone defects were made on both sides. In the UV group (n = 30), the defects were covered with uHA/PLLA mesh, treated by UV (wavelength 172 nm, tube wall illumination 13 mW/cm2, period 8 min, intensity 6.26 J/cm2) and screwed. In the control group (n = 30), untreated uHA/PLLA was applied in a similar manner. The rabbits were sacrificed at 1, 2, 3, 4 and 6 weeks postoperatively, and formalin-fixed specimens were frozen. The specimens were stained with haematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with anti-alkaline phosphatase (ALP). Finally, bone ratio and ALP expression were evaluated microscopically.
Results:
The UV group had a significantly higher number of ALP stained cells than the control group after 1, 2, and 3 weeks (P < 0.05). The bone ratio was also significantly higher in the UV group than in the control group after 1, 2, 3, and 4 weeks (P < 0.05).
Conclusion:
This study suggests that bone regeneration can be enhanced by UV treatment using an uHA/PLLA mesh.
