Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From mechanism to substratome: unraveling mysteries of γ-secretase.

The Journal of biological chemistry·2026

Same author

Current status of human endogenous retrovirus annotation.

Briefings in bioinformatics·2026

Same author

Multi-platform integration of brain and CSF proteomes reveals biomarker panels for Alzheimer's disease.

Briefings in bioinformatics·2026

Same author

BLMPred: Predicting linear B-cell epitopes using pre-trained protein language models and machine learning.

Computational and structural biotechnology journal·2026

Same author

Structural and functional effects of disease mutations in the interface of human membrane protein complexes.

International journal of biological macromolecules·2025

Same author

The EMC acts as a chaperone for membrane proteins.

Nature communications·2025

Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026

Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026

Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026

Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026

Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026

Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 18, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Melting temperature highlights functionally important RNA structure and sequence elements in yeast mRNA coding

Fei Qi¹, Dmitrij Frishman^1,2

¹Department of Bioinformatics, Technische Universität München, Wissenschaftzentrum Weihenstephan, Maximus-von-Imhof-Forum 3, D-85354 Freising, Germany.

Nucleic Acids Research

|March 24, 2017

Summary

This summary is machine-generated.

Regions of messenger RNA (mRNA) with high melting temperatures (Tm) preserve RNA structure, while low Tm regions conserve RNA sequence. This highlights distinct evolutionary pressures on mRNA functional elements.

More Related Videos

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 14, 2014

Related Experiment Videos

Last Updated: Dec 18, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 14, 2014

Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

Secondary structures in mRNA coding regions are crucial for gene expression and regulation.
Differentiating functional from non-functional RNA structures is a significant challenge.

Purpose of the Study:

To investigate how temperature influences sequence-structure relationships in mRNA coding regions.
To analyze evolutionary pressures on mRNA regions with varying thermostability.

Main Methods:

Analysis of recent PARTE data.
Comparison of evolutionary conservation and synonymous constraint in high and low melting temperature (Tm) regions.
Evaluation of theoretical predictions versus experimental measurements of mRNA structures at different temperatures.

Main Results:

High Tm regions are under pressure to maintain RNA secondary structure, allowing relaxed sequence constraint.
Low Tm regions are under pressure to conserve primary sequence, showing higher evolutionary conservation and synonymous constraint.
Theoretically predicted mRNA structures converge with increasing temperature, while experimentally measured structures diverge.

Conclusions:

High Tm regions likely harbor stable, functionally important RNA structures.
Low Tm regions likely contain single-stranded, conserved RNA sequence elements for molecular interactions.
Current computational models may not fully capture RNA structure melting pathways.