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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Maxam-Gilbert Sequencing01:05

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Nanopore DNA Sequencing for Metagenomic Soil Analysis
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DNA Sequencing Sensors: An Overview.

Jose Antonio Garrido-Cardenas1, Federico Garcia-Maroto2, Jose Antonio Alvarez-Bermejo3

  • 1Department of Biology and Geology, University of Almeria, 04120 Almeria, Spain. jcardena@ual.es.

Sensors (Basel, Switzerland)
|March 25, 2017
PubMed
Summary

DNA sequencing sensors have evolved significantly over 40 years, from Sanger sequencing to new generation sequencing (NGS) and beyond. Future technologies promise cheaper, longer DNA reads through miniaturized, precise sensors.

Keywords:
DNA sequencingfluorescencenanoporenext generation sequencing (NGS)pyrosequencingsemiconductor

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Area of Science:

  • Genomics
  • Biotechnology
  • Nanotechnology

Background:

  • The first complete genome sequencing, by Frederick Sanger, utilized early technology.
  • Advances in biochemistry, molecular genetics, nanotechnology, and computing have driven progress.
  • Modern DNA sequencing sensors are vastly different from early methods.

Purpose of the Study:

  • To provide a comprehensive overview of DNA sequencing sensors developed over the past 40 years.
  • To highlight the technological evolution in DNA sequencing.
  • To discuss the impact of sensor advancements on sequencing capabilities.

Main Methods:

  • Review of historical and current DNA sequencing technologies.
  • Analysis of advancements in sensor technology and detection systems.
  • Discussion of emerging fourth-generation sequencing approaches.

Main Results:

  • The emergence of new generation sequencing (NGS) marked a significant leap in sequencing volume, speed, and cost-effectiveness.
  • Continuous innovation in sensor miniaturization and precision is crucial for sequencing advancements.
  • Fourth-generation sequencing technologies are expected to further reduce costs and increase DNA read lengths.

Conclusions:

  • DNA sequencing technology has undergone a dramatic transformation in the last four decades.
  • Sensor and detection system improvements are fundamental to the progress of DNA sequencing.
  • Future sequencing technologies will likely offer even greater efficiency and affordability.