Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Protein Translocation Machinery on the ER Membrane

Protein Translocation Machinery on the ER Membrane

The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Cotranslational Protein Translocation

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...

Ribosomes

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Can understanding the spatial expression of IL-33 and its receptor ST2 in the lower airways inform clinical studies?

American journal of respiratory and critical care medicine·2026

Same author

Longitudinal <sup>1</sup>H and <sup>129</sup>Xe Lung MRI in Patients With Post-COVID Residual Lung Abnormalities.

Journal of magnetic resonance imaging : JMRI·2026

Same author

Genome-wide association study of Idiopathic Pulmonary Fibrosis susceptibility using clinically-curated European-ancestry datasets.

The European respiratory journal·2026

Same author

Elucidating the Association Between DNA Methylation and COPD in Bronchial Biopsies.

Archivos de bronconeumologia·2026

Same author

The crucial role of normative ageing in respiratory pathogenesis.

The lancet. Healthy longevity·2026

Same author

A comprehensive multi-omics and functional study of evolutionary adaptive responses to smoke.

iScience·2026

Same journal

Tissue identity is the dominant determinant of cross-species transferability of a porcine developmental programme.

BMC genomics·2026

Same journal

Characterization of mitochondrial genomes from three medicinal species of rutaceae and comparative analysis within the family: insights into evolution.

BMC genomics·2026

Same journal

Comparative genomic analysis of Herbaspirillum strains associated with banana sheath rot reveals potential virulence factors.

BMC genomics·2026

Same journal

Transposable element disruption of a second thyroglobulin-like gene confers Vip3Aa resistance in Helicoverpa armigera.

BMC genomics·2026

Same journal

Molecular ontogeny of Bemisia tabaci life stages reveals miRNA-governed development and egg-stalk support for early embryogenesis.

BMC genomics·2026

Same journal

Genome-wide characterization of the accessible chromatin regions in chickpea.

BMC genomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 5, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Lung function associated gene Integrator Complex subunit 12 regulates protein synthesis pathways.

Alexander K Kheirallah^1,2, Cornelia H de Moor³, Alen Faiz⁴

¹Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. akk43@cam.ac.uk.

|March 25, 2017

Summary

This summary is machine-generated.

The Integrator Complex subunit 12 (INTS12) gene regulates protein synthesis and airway biology, impacting human lung function. This finding clarifies the genetic basis for lung function at the 4q24 locus.

Keywords:

Accessible chromatin Histone modification INTS12 Integrator Complex Pathway dysregulation Protein synthesis Regulation of gene expression Transcription snRNA processing

More Related Videos

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Published on: May 10, 2018

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

Related Experiment Videos

Last Updated: Mar 5, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Published on: May 10, 2018

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

Area of Science:

Genetics
Molecular Biology
Pulmonary Medicine

Background:

Genetic studies identified a significant locus (4q24) associated with human lung function and Chronic Obstructive Pulmonary Disease.
Two candidate genes at this locus, GSTCD and INTS12, were investigated for their role in lung function regulation.
INTS12, a component of the Integrator Complex, was previously thought to primarily mediate small nuclear RNA processing.

Purpose of the Study:

To investigate the function of INTS12 in lung tissue and its role in regulating lung function.
To determine whether INTS12 or GSTCD is the primary gene responsible for the lung function signal at 4q24.
To elucidate the genome-wide activities of INTS12 beyond its canonical function.

Main Methods:

Analysis of lung tissue polymorphisms correlating with gene expression.
INTS12 depletion studies and assessment of small nuclear RNA processing.
RNA sequencing (RNAseq) and ChIP-sequencing (ChIPseq) to analyze gene expression and genome-wide binding.
Identification of the INTS12 regulome.

Main Results:

Polymorphisms at 4q24 associated with lung function correlated with INTS12 expression, not GSTCD expression, in lung tissue.
INTS12 depletion caused only minor alterations in small nuclear RNA processing, contrary to previous reports.
RNAseq revealed dysregulation of airway biology genes and significant downregulation of protein synthesis pathways upon INTS12 knockdown.
ChIPseq demonstrated INTS12 binding across the genome, particularly in active transcription regions, and identified protein synthesis pathway genes as part of the INTS12 regulome.

Conclusions:

INTS12 possesses functions beyond canonical small nuclear RNA processing.
INTS12 regulates translation through the modulation of protein synthesis pathway gene expression.
This study provides a comprehensive genome-wide analysis of INTS12 activities, contributing to the understanding of the genetic basis for lung function at 4q24.