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A novel sensitive amperometric choline biosensor based on multiwalled carbon nanotubes and gold nanoparticles
Hend Samy Magar1, Mariana Emilia Ghica2, Mohammed Nooredeen Abbas3
1Applied Organic Chemistry Department, National Research Centre, Giza, Egypt; Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal.
Talanta
|March 26, 2017
Summary
A novel biosensor using multiwalled carbon nanotubes and gold nanoparticles offers sensitive choline detection. This advancement enables precise measurements in complex samples like milk.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biotechnology
Background:
- Choline determination is crucial for various applications.
- Developing sensitive and selective biosensors is an ongoing challenge.
- Nanomaterials offer unique properties for biosensor development.
Purpose of the Study:
- To develop a novel amperometric biosensor for choline determination.
- To utilize the electrocatalytic properties of multiwalled carbon nanotubes (MWCNT) and gold nanoparticles (GNP).
- To explore electrochemical impedance spectroscopy (EIS) for choline measurement.
Main Methods:
- Fabrication of a biosensor by immobilizing choline oxidase (ChOx) on a glassy carbon electrode (GCE) modified with chitosan-dispersed MWCNT and GNP.
- Electrochemical characterization using cyclic voltammetry and amperometry.
- Electrochemical impedance spectroscopy (EIS) for choline detection.
- Validation of the biosensor in milk samples.
Main Results:
- The developed ChOx/(GNP)4/MWCNT/GCE biosensor showed a linear response to choline from 3 to 120µM.
- Achieved a high sensitivity of 204µAcm-2mM-1 and a low detection limit of 0.6μM.
- Demonstrated excellent selectivity, stability, and precision.
- Successfully measured choline in milk samples using both amperometry and EIS.
Conclusions:
- A novel and efficient amperometric biosensor for choline determination has been successfully developed.
- The combination of MWCNT, GNP, and ChOx provides enhanced electrocatalytic activity and sensitivity.
- The biosensor exhibits promising performance for practical applications, including analysis in real-world samples like milk.
