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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Related Experiment Video

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Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
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Rapid micromotor-based naked-eye immunoassay.

Berta Esteban-Fernández de Ávila1, Mingjiao Zhao2, Susana Campuzano3

  • 1Department of Nanoengineering,, University of California, La Jolla, San Diego, CA 92093, United States.

Talanta
|March 26, 2017
PubMed
Summary

This study introduces a novel micromotor-based immunoassay for rapid and sensitive cortisol detection. Antibody-functionalized micromotors accelerate assays and enhance sensitivity, enabling quick, visual results.

Keywords:
AntibodyCortisolMicromotorNaked-eye detectionReal-time sensing

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Immunoassays are crucial for detecting biomarkers like cortisol.
  • Traditional immunoassays can be time-consuming and lack sensitivity.
  • Micromotor technology offers potential for assay acceleration and enhanced detection.

Purpose of the Study:

  • To develop a dynamic micromotor-based immunoassay for cortisol detection.
  • To enhance the speed and sensitivity of cortisol immunoassays.
  • To enable rapid, visual, and on-the-move detection of cortisol.

Main Methods:

  • Utilized tubular micromotors functionalized with specific antibodies.
  • Employed a horseradish peroxidase (HRP) tag with a TMB/H2O2 system for visual detection.
  • Optimized key parameters of the competitive immunoassay, including incubation time and reaction volume.

Main Results:

  • Achieved significant acceleration in both direct and competitive cortisol immunoassays.
  • Demonstrated greatly enhanced sensitivity for cortisol detection.
  • Enabled rapid, naked-eye detection of cortisol down to 0.1μgmL-1 in just 2 minutes.
  • Utilized ultrasmall sample volumes (50µL).

Conclusions:

  • Micromotor-based immunoassays offer a highly efficient and rapid recognition process.
  • This approach provides a fast visual sensing platform for "on the move" specific detection.
  • The technology significantly improves upon traditional immunoassay limitations in speed and sensitivity.