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IL-23 Gene and Protein Expression in Childhood Asthma
Soheila Alyasin1, Reza Amin, Ali Fazel
1Department of Allergy and Clinical Immunology, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Iranian Journal of Immunology : IJI
|March 26, 2017
Summary
Interleukin-23 (IL-23) serum levels and gene expression were significantly elevated in children with persistent asthma compared to healthy controls. This suggests IL-23 may serve as a diagnostic biomarker for pediatric asthma.
Area of Science:
- Pediatric Pulmonology
- Immunology
- Biomarker Discovery
Background:
- Asthma involves chronic airway inflammation driven by T helper 2 cell cytokines.
- Interleukin-23 (IL-23) is increasingly recognized for its role in adult allergic asthma pathogenesis.
Purpose of the Study:
- To investigate serum IL-23 levels and gene expression in young children with persistent asthma.
- To compare these levels against a control group of healthy children.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR were used.
- Blood samples from 40 children with persistent asthma (mild/severe) and 34 healthy children were analyzed.
Main Results:
- Significantly higher IL-23 gene expression was observed in children with persistent asthma versus controls (p=0.001).
- IL-23 serum levels were also significantly elevated in asthmatic children compared to controls (p=0.002).
- No significant difference in IL-23 gene expression was found between mild and severe asthma groups.
Conclusions:
- Elevated IL-23 serum levels show potential as an auxiliary diagnostic biomarker for asthma in pre-school children.
- This is particularly relevant when spirometry testing is not feasible.
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