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Is a decrease in cyclic AMP a necessary and sufficient signal for maturation of amphibian oocytes?

S Gelerstein1, H Shapira, N Dascal

  • 1Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Israel.

Developmental Biology
|May 1, 1988
PubMed
Summary
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Acetylcholine and adenosine influence Xenopus oocyte maturation by altering cyclic AMP levels and calcium release. Adenosine alone can induce maturation, suggesting calcium signaling plays a key role.

Area of Science:

  • Reproductive biology
  • Cell signaling
  • Developmental biology

Background:

  • Cyclic AMP (cAMP) plays a crucial role in regulating oocyte maturation.
  • Acetylcholine and adenosine are signaling molecules known to affect cellular processes.

Purpose of the Study:

  • To investigate the effects of acetylcholine and adenosine on Xenopus laevis oocyte maturation.
  • To elucidate the roles of intracellular cyclic AMP and calcium in these processes.

Main Methods:

  • Treatment of Xenopus oocytes (stages 5 and 6) with acetylcholine and adenosine.
  • Measurement of intracellular cyclic AMP levels.
  • Assessment of oocyte maturation induction and acceleration.
  • Analysis of 45Ca efflux rates.

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Main Results:

  • Acetylcholine rapidly decreased intracellular cAMP levels and accelerated progesterone-induced maturation, independent of extracellular calcium.
  • Adenosine increased cAMP, abolished progesterone's effect on cAMP, and induced/accelerated maturation.
  • Adenosine also increased 45Ca efflux, even without external calcium.

Conclusions:

  • Oocyte maturation can be induced or accelerated by activating cell surface receptors that release calcium from internal stores.
  • These effects may occur independently of significant changes in intracellular cyclic AMP concentration.