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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Mar 5, 2026

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Focus: The interface between data collection and data processing in cryo-EM.

Nikhil Biyani¹, Ricardo D Righetto¹, Robert McLeod¹

¹Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.

Journal of Structural Biology

|March 28, 2017

Summary

This summary is machine-generated.

Focus is a new software package for cryo-transmission electron microscopy (cryo-EM) that integrates data collection and image processing. It streamlines high-throughput data management and processing, enabling remote monitoring and rapid error detection for increased microscope productivity.

Keywords:

Automated data collection Cryo-EM Drift correction High throughput image processing

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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

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Related Experiment Videos

Last Updated: Mar 5, 2026

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Area of Science:

Microscopy
Computational Biology
Structural Biology

Background:

Automated cryo-transmission electron microscopy (cryo-EM) enables high-throughput data acquisition.
Integrating data collection with image processing is crucial for efficient cryo-EM workflows.
Real-time monitoring and error detection are essential for maximizing microscope productivity.

Purpose of the Study:

Introduce Focus, a novel software package for cryo-EM.
Provide a user-friendly interface for data management and image processing.
Facilitate remote monitoring of data collection and processing.

Main Methods:

Focus software package developed to interface cryo-EM data collection with computer image processing.
User-friendly environment for importing and managing data from direct electron detectors.
High-throughput elemental image processing tasks performed concurrently with data acquisition.

Main Results:

Focus enables seamless integration of cryo-EM data collection and processing.
Remote monitoring functionality allows for efficient oversight of overnight data collection sessions.
Rapid error detection capabilities significantly enhance the productivity of cryo-EM experiments.

Conclusions:

Focus software package enhances cryo-EM workflow efficiency.
The software facilitates high-throughput processing and remote monitoring of data.
Focus is a valuable tool for increasing productivity in cryo-EM studies.