Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Update of the <i>Xylella</i> spp. host plant database - Systematic literature search up to 31 December 2025.

EFSA journal. European Food Safety Authority·2026
Same author

Intron location and sequence modulate gene expression in Yarrowia lipolytica.

Nucleic acids research·2026
Same author

CEN-Display: Construction and optimization of a surface display system in Saccharomyces cerevisiae CEN.PK2-1C.

Bioresource technology·2026
Same author

Next-generation brewing yeasts for non-alcoholic beers.

Current opinion in biotechnology·2026
Same author

Designing the future of food fats: precision fermentation of Yarrowia lipolytica for tailored lipid production.

Current opinion in biotechnology·2026
Same author

Beyond natural evolution: multi-scale in vivo mutagenesis toolkits for synthetic evolution.

Trends in biotechnology·2026

Related Experiment Video

Updated: Mar 5, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.3K

High-throughput system-wide engineering and screening for microbial biotechnology.

Yannick Vervoort1, Alicia Gutiérrez Linares2, Miguel Roncoroni2

  • 1Laboratory for Systems Biology, VIB Center for Microbiology, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Laboratory for Genetics and Genomics, KU Leuven, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Imec Life Science Technologies, Kapeldreef 75, B-3001 Leuven, Belgium.

Current Opinion in Biotechnology
|March 28, 2017
PubMed
Summary

High-throughput genome engineering and screening methods accelerate microbial systems biology. Technologies like multiplex automated genome evolution (MAGE) and CRISPR/Cas, coupled with nanoreactors, enable rapid, large-scale analysis at the single-cell level.

More Related Videos

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.5K
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

7.6K

Related Experiment Videos

Last Updated: Mar 5, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.3K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.5K
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

7.6K

Area of Science:

  • Microbiology
  • Systems Biology
  • Synthetic Biology

Background:

  • High-throughput screening and genetic engineering are essential for advancing systems biology and applied microbiology.
  • Traditional methods using bottles and plates are often slow and inefficient for large-scale experiments.
  • Miniaturization to nanoliter/picoliter scales and single-cell analysis are key trends in modern biological research.

Purpose of the Study:

  • To review current high-throughput genome engineering and screening strategies for microbial applications.
  • To highlight the utility of multiplex automated genome evolution (MAGE) and CRISPR/Cas systems.
  • To discuss the application of nanoreactors for high-throughput single-cell and population screening.

Main Methods:

  • Review of literature on high-throughput microbial genome engineering and screening.
  • Focus on multiplex automated genome evolution (MAGE) for genome engineering.
  • Emphasis on CRISPR/Cas systems for precise genome modification.
  • Discussion of (lab-on-chip) nanoreactors for miniaturized screening.

Main Results:

  • High-throughput strategies significantly overcome bottlenecks in genetic engineering and screening.
  • Multiplex automated genome evolution (MAGE) and CRISPR/Cas enable efficient, large-scale genome engineering.
  • Nanoreactor technology facilitates high-throughput screening at the single-cell and population levels.
  • These integrated approaches accelerate discovery in microbial systems biology.

Conclusions:

  • High-throughput genome engineering and screening are revolutionizing microbial research.
  • The combination of MAGE, CRISPR/Cas, and nanoreactors offers powerful tools for systems and applied microbiology.
  • These advanced techniques are crucial for understanding and manipulating microbial systems at an unprecedented scale.