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Arylamine N-acetyltransferase 2 gene polymorphism in an Algerian population
Hiba Chelouti1, Malika Khelil1
1a Département de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene , Alger ; Algérie.
Background:
The arylamine N-acetyltransferase 2 (NAT2) is a key enzyme in the biotransformation of xenobiotics. NAT2 gene polymorphisms have been associated with the risk of isoniazid hepatotoxicity and these polymorphisms change among different populations.
Aim:
The objective of this study is to investigate NAT2 polymorphisms in order to predict the prevalence of NAT2 phenotype in an Algerian population.
Subjects And Methods:
Genotyping of NAT2 was done using a PCR-RFLP method. Haplotype was analysed using the software package PHASE, version 2.0.
Results:
The major haplotypes were NAT2*5B (23.72%), NAT2*6 A (18.61%), NAT2*4 (14.60%) and NAT2*5 F (10%). The average of the expected slow acetylator phenotype was 53%.
Conclusion:
Our results suggest that the high frequency of slow acetylator phenotype requires investigation into its possible association with ATDH.
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