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Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
Published on: June 13, 2018
3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches
Vitalii V Mutsenko1, Vasilii V Bazhenov2, Olena Rogulska3
1Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany.
Abstract:
The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.
