Related Experiment Video
Updated: Mar 5, 2026
13:42
RNA Secondary Structure Prediction Using High-throughput SHAPE
Published on: May 31, 2013
32.3K
In Silico Prediction of RNA Secondary Structure
Fariza Tahi1,2, Van Du T Tran3, Anouar Boucheham4,5
1IBISC, UEVE/Genopole, 23 bv. de France, 91000, Evry, France. fariza.tahi@ibisc.univ-evry.fr.
Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2017
Summary
This chapter reviews RNA secondary structure prediction tools and noncoding RNA identification methods. It highlights microRNA identification and their role in gene regulation and human diseases.
Area of Science:
- Molecular Biology
- Bioinformatics
Background:
- RNA secondary structure dictates molecular function.
- Noncoding RNAs, including microRNAs, are crucial regulators of gene expression.
- Dysregulation of microRNAs is implicated in various human diseases.
Purpose of the Study:
- To provide an overview of RNA secondary structure prediction tools.
- To discuss methods for identifying noncoding RNAs from genomic and RNA sequencing data.
- To focus on the identification and significance of microRNAs.
Main Methods:
- Review of computational approaches for RNA secondary structure prediction.
- Exploration of algorithms for noncoding RNA identification.
- Discussion of microRNA identification strategies.
Main Results:
- Established computational tools exist for predicting RNA secondary structures.
- Various methods are available for detecting noncoding RNAs in genomic data.
- MicroRNA identification is a key area with significant biological implications.
Conclusions:
- Accurate RNA secondary structure prediction is fundamental to understanding RNA function.
- Effective identification of noncoding RNAs is essential for biological research.
- MicroRNAs are vital regulators with critical roles in health and disease.
Related Concept Videos
01:19RNA Structure
8.0K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.0K
01:23RNA Structure
79.7K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.7K
03:21RNA-seq
12.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.3K
01:25Nucleic Acid Structure
9.7K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
DNA Structure
DNA...
9.7K
01:53RNA Stability
36.0K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
36.0K
01:23RNA Interference
28.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.3K