Related Experiment Video
Updated: Mar 5, 2026

13:47
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
26.5K
Statistical Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite Sequencing
1Department of Biology, New York University, 100 Washington Sq East, New York, NY, 10003, USA. brian.parker@nyu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2017
Abstract:
For the transcriptome-wide detection and quantification of the 5-methylcytosine (m5C) methylation modification of RNA, one experimental approach is via bisulfite conversion. In this chapter we discuss statistical methods, and a corresponding computational pipeline, to perform transcriptome-wide differential m5C methylation analysis between RNA samples, specialized for this assay.

