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Updated: Mar 5, 2026
Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
A rapid, simple and sensitive loop-mediated isothermal amplification method to detect Anaplasma bovis in sheep and
Jinhong Wang1, Yan Zhang1, Yanyan Cui1
1Parasitology Laboratory, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China.
Abstract:
A loop-mediated isothermal amplification (LAMP) technique has been widely used in detecting the nucleic acid of various pathogenic bacteria. In this study, a set of four LAMP primers was designed to specifically test Anaplasma bovis. The LAMP assay was performed at 62°C for 60min in a water bath. The specificity was confirmed by amplifying A. bovis isolate, while no cross reaction was observed with other five pathogens (Anaplasma bovis, Anaplasma phagocytophilum, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum). The sensitivity of LAMP was 5×100copies/μL, 100 times more than that of conventional PCR (5×102copies/μL). Of 120 blood DNA extracted from sheep and goats field samples, 81 (67.5%), 22 (18.3%) and 43 (35.8%) were positively detected by LAMP, conventional PCR and nested PCR, respectively. The findings indicated that the developed LAMP assay is a new convenient tool for rapid and cost-effective detection of A. bovis.
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