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Updated: Mar 5, 2026

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Published on: July 7, 2013
Proteomic Profiling of Skin Fibroblasts as a Model of Schizophrenia
Lan Wang1, Hassan Rahmoune2, Paul C Guest3
1Apitope Technology (Bristol) Ltd, Riverside Court, Beaufort Park, Chepstow, NP16 5UH, UK.
Abstract:
Since many aspects of schizophrenia are also manifested at the peripheral level in proliferating cell types, this chapter describes the analysis of skin fibroblasts biopsied from living patients. The method focuses on cell culture and sample preparation for characterization of the model. The resulting cell extracts can be analysed by any number of proteomic techniques for identification of biomarker candidates. This approach could help to elucidate the molecular mechanisms associated with the pathophysiology of schizophrenia and provide a useful model for a new target and drug discovery.
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