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Author Spotlight: Advancements in iPSCs and Genetic Disease Research
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Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium
Guokai Chen1,2, Mahendra Rao3,4,5
1Faculty of Health Sciences, University of Macau, Taipa, Macau, China. GuokaiChen@umac.edu.
Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2017
Abstract:
Human somatic cells can be reprogrammed by defined factors to induced pluripotent stem cells (iPSCs). Importantly, the quality of iPSCs could impact the potential of these cells in basic and clinic research. Here, we describe a method to reprogram human fibroblast cells with Sendai virus in chemically defined conditions, to generate iPSCs that are integration-free and suitable for research and translational applications.
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