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Cryobanking Pluripotent Stem Cells.

Jeremy M Crook1,2,3, Eva Tomaskovic-Crook4,5, Tenneille E Ludwig6

  • 1ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia. jcrook@uow.edu.au.

Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2017
PubMed
Summary
This summary is machine-generated.

Cryopreservation of human pluripotent stem cells (hPSCs) is crucial for research and therapies. This chapter details reliable methods for hPSC cryopreservation, ensuring quality for diverse applications.

Keywords:
BankingControl rate freezingCryopreservationHuman pluripotent stem cellsVitrification

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Area of Science:

  • Stem Cell Biology
  • Cryobiology
  • Regenerative Medicine

Background:

  • Cryopreservation of human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), is vital for their application in research and cell-based therapies.
  • Established cell banking requires robust quality assurance during freezing and storage.
  • Existing cryopreservation protocols, including vitrification and controlled-rate freezing, show variable efficacy.

Purpose of the Study:

  • To present standardized and reliable protocols for hPSC cryopreservation.
  • To detail methods suitable for routine research, high-quality investigations, and clinical compliance.
  • To address the need for consistent and effective cryopreservation techniques in the field.

Main Methods:

  • Review and detailing of various hPSC cryopreservation techniques.
  • Discussion of methods ranging from conventional vitrification to advanced controlled-rate freezing.
  • Emphasis on protocols adaptable for different research and clinical requirements.

Main Results:

  • Identification of multiple effective cryopreservation methods for hPSCs.
  • Demonstration of protocols suitable for generating working and master cell banks with quality assurance.
  • Highlighting the adaptability of methods for basic science, applied research, and clinical development.

Conclusions:

  • Standardized cryopreservation protocols are essential for advancing hPSC research and therapeutics.
  • The presented methods offer reliability and adaptability for diverse applications.
  • Effective cryopreservation ensures the long-term viability and utility of hPSCs for scientific and clinical purposes.