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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

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Related Experiment Video

Updated: Mar 5, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

Cryobanking Mesenchymal Stem Cells.

Andrés Pavón¹, Izaskun Beloqui¹, Juan M Salcedo¹

¹StemTek Therapeutics, Derio, 48160, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|March 30, 2017

Summary

This summary is machine-generated.

Cryopreservation of mesenchymal stem cells (MSCs) is vital for biobanks. Optimized protocols ensure viable MSCs for research and clinical applications by controlling freezing rates and storage conditions.

Keywords:

Control rate freezing Cryopreservation Cryoprotectant DMSO Mesenchymal stem cells Viability

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Area of Science:

Cell Biology
Biotechnology
Regenerative Medicine

Background:

Mesenchymal stem cells (MSCs) are crucial for research and clinical applications.
Maintaining viable MSCs in biobanks requires effective cryopreservation.
Existing protocols need optimization to minimize cell damage during freezing and thawing.

Purpose of the Study:

To describe optimized protocols for cryopreserving culture-expanded mesenchymal stem cells (MSCs).
To ensure the reliable availability of viable MSCs for biobanking purposes.
To minimize cell damage during the cryopreservation process.

Main Methods:

Controlled freezing rates.
Selection of appropriate cryoprotective agents.
Maintenance of stable storage temperatures.

Main Results:

Protocols minimize damage to MSCs during freezing and thawing.
Viable MSCs are reliably available for research and clinical use.
Optimized cryopreservation enhances the long-term utility of MSC biobanks.

Conclusions:

Effective cryopreservation protocols are essential for MSC biobanking.
Controlling freezing rates, cryoprotectant use, and storage temperature are key factors.
Optimized methods ensure the integrity and viability of MSCs for future applications.