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Assessment of Kidney Function in Mouse Models of Glomerular Disease
Published on: June 30, 2018
Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice
K MacKay1, L J Striker, S Elliot
1Metabolic Disease Branch, National Institute of Diabetes, Digestive and Kidney Disease, Bethesda, Maryland.
Kidney International
|March 1, 1988
Summary
Researchers created permanent glomerular cell lines from mice engineered with simian virus 40 (SV40). These cell lines offer a new tool for studying glomerulosclerosis and normal glomerular cell biology.
Area of Science:
- Nephrology
- Cell Biology
- Genetics
Background:
- Glomerular cell culture is crucial for understanding kidney function.
- Obtaining pure glomerular cell populations and culturing endothelial cells is challenging.
- Currently, no established glomerular cell lines exist.
Purpose of the Study:
- To establish permanent cell lines of cloned glomerular epithelial, mesangial, and endothelial cells.
- To provide tools for studying glomerulosclerosis pathogenesis.
- To investigate normal glomerular cell biology in transformed cells.
Main Methods:
- Utilized mice transgenic for the early region of simian virus 40 (SV40).
- Established permanent cell lines from cloned glomerular epithelial, mesangial, and endothelial cells.
- Characterized cells for features of their normal counterparts despite transformation.
Main Results:
- Successfully established permanent, cloned cell lines of glomerular epithelial, mesangial, and endothelial cells.
- Transgenic mice developed glomerulosclerosis by 3-4 months.
- The established cell lines retained characteristics of normal glomerular cells.
Conclusions:
- Permanent cell lines of glomerular cells have been successfully created.
- These cell lines are valuable for studying glomerulosclerosis in the transgenic model.
- The cell lines can aid in understanding normal glomerular cell biology.
